Evaluation of different detection modes for the analysis of procyanidins in leaves and flowers of <i>Crataegus</i> spp.: Part I.: Diode array and electrochemical detection

作     者：Rohr, GE Meier, B Sticher, O 

作者机构：ETH Zurich Dept Pharm CH-8057 Zurich Switzerland Zeller AG CH-8590 Romanshorn Switzerland 

出 版 物：《PHYTOCHEMICAL ANALYSIS》 (植物化学分析)

年 卷 期：2000年第11卷第2期

页      面：106-112页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 0703[理学-化学] 

主　　题：liquid chromatography electrochemical detection diode array detection flavan-3-ols procyanidins Crataegus 

摘      要：Diode array and single electrode electrochemical detection were evaluated for their use in the analysis of (-)-epicatechin and oligomeric procyanidins in reference solutions and extracts of leaves and flowers of Crataegus spp, Linearity of regression curves was good for both detection modes. Diode array detection was found to be more selective but less sensitive than electrochemical detection, The absorbance ratio 220/280 nm gives information on the degree of polymerization in reference solutions, In the presence of matrix no additional structural information can be obtained from the absorbance ratios due to high standard deviations. Quantitative evaluation in terms of monomer equivalents using electrochemical detection leads to an underestimation of the contents of procyanidin dimers, but it is applicable using detection at 280 nm, in view of the better selectivity and the easier handling, diode array detection is recommended for the analysis of procyanidins in complex matrices. Copyright (C) 2000 John Wiley & Sons, Ltd.