Subcellular alterations induced by UV-oxidized low-density lipoproteins in epithelial cells can be counteracted by α-tocopherol

作     者：Masella, R Straface, E Giovannini, C Di Benedetto, R Scazzocchio, B Viora, M Cantafora, A Malorni, W 

作者机构：Ist Super Sanita Dept Metab & Pathol Biochem I-00161 Rome Italy Ist Super Sanita Dept Ultrastruct I-00161 Rome Italy Ist Super Sanita Dept Foods I-00161 Rome Italy Ist Super Sanita Dept Immunol I-00161 Rome Italy 

出 版 物：《PHOTOCHEMISTRY AND PHOTOBIOLOGY》 (Photochem. Photobiol.)

年 卷 期：2000年第71卷第1期

页      面：97-102页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

主　　题：上皮细胞/药物作用 上皮细胞/代谢 上皮细胞/超微结构 脂蛋白类 LDL/生理学 脂蛋白类 LDL/辐射效应 显微镜检查 电子 扫描 氧化性应激 亚细胞部分/药物作用 亚细胞部分/代谢 肿瘤细胞 培养的 紫外线 维生素E/药理学 人类 

摘      要：Oxidized LDL (ox-LDL) have been involved in the pathogenesis of several human diseases including dermatological pathologies, Oxidative modification of low-density lipoproteins (LDL) is accompanied by both extensive degradation of its polyunsaturated fatty acids and production of lipoperoxides, These highly reactive products induce an intracellular oxidative stress with a variety of cytotoxic effects. In order to evaluate cellular damage induced by oxidative stress in epidermal cells, a human epidermoid carcinoma cell line in culture (A 431) was used as experimental model. Cell treatment with W-oxidized LDL resulted in cytostatic and cytotoxic effects characterized by morphological and functional alterations: inhibition of cell proliferation, modifications of cytoskeleton network, microtubular derangement, loss of cell-cell and cell-substrate contacts, cell detachment and cell death by apoptosis, The ox-LDL-induced alterations were almost completely prevented by pre-incubating cells with alpha-tocopherol. The results presented here could be of relevance for a better comprehension of the pathogenic mechanisms of several human diseases, including dermatological pathologies, and could indicate that antioxidants such as ar-tocopherol could represent an important therapeutic challenge in the maintenance of cell and tissue homeostasis in the long run.