Point spread function of optical microscopes imaging through stratified media

作     者：Haeberlé, O Ammar, M Furukawa, H Tenjimbayashi, K Török, P 

作者机构：Univ Haute Alsace Grp Lab E1 Lab MIPS F-68093 Mulhouse France AIST OptMec Photon Res Inst Tsukuba Ibaraki 3058564 Japan Univ London Imperial Coll Sci Technol & Med Blackett Lab London SW7 2BW England 

出 版 物：《OPTICS EXPRESS》 (Opt. Express)

年 卷 期：2003年第11卷第22期

页      面：2964-2969页

核心收录：

学科分类：070207[理学-光学] 07[理学] 08[工学] 0803[工学-光学工程] 0702[理学-物理学] 

主　　题：.aperture specimen Point Spread STRATIFIED fluorescence microscopy optical microscopes 

摘      要：We propose a model for imaging point objects through a dielectric interface or stratified media. The model is applicable to conventional and confocal fluorescence microscopy, with single- or multi-photon excitation. An analytical solution is obtained in the form of readily computable functions. When large mismatches occur in the refractive indices of the media of the objective lens and specimen the illumination and detection point spread functions differ significantly, showing that currently used imaging models may fail to correctly predict imaging properties of optical microscopes. (C) 2003 Optical Society of America.