Anti-inflammatory activity of dichloromethane extract of <i>Heterotheca inuloides in vivo</i> and <i>in vitro</i>

作     者：Segura, L Freixa, B Ringbom, T Vila, R Perera, P Adzet, T Bohlin, L Cañigueral, S 

作者机构：Univ Barcelona Fac Farm Unitat Farmacol & Farmacognosia E-08028 Barcelona Spain Uppsala Univ Ctr Biomed Dept Pharm Div Pharmacognosy Uppsala Sweden 

出 版 物：《PLANTA MEDICA》 (药用植物)

年 卷 期：2000年第66卷第6期

页      面：553-555页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 10[医学] 

主　　题：菊科/化学 细胞 培养的 环加氧酶抑制药/化学 环加氧酶抑制药/分离和提纯 环加氧酶抑制药/药理学 植物提取物/化学 植物提取物/分离和提纯 植物提取物/药理学 人类 小鼠 

摘      要：The dichloromethane extract from the dried flowers of Heterotheca inuloides Cass. was investigated on several pharmacological models of inflammation in vivo and in vitro. It showed anti-inflammatory activity on the croton oil-induced oedema test in mouse ear, at 1 mg/ear. The compound isolated from this extract, 7-hydroxy-3,4-dihydrocadalin, showed antiinflammatory effect on the same experimental model (ED50 of 0.9 mu mol/ear), as well as on COX-1 and COX-2 catalysed prostaglandin biosynthesis assays, with IC50 values of 22 mu M and 526 mu M, respectively. No effect was observed on carrageenan-induced oedema and on fMLP/PAF-induced exocytosis of human neutrophils. The COX-1 inhibitory effect showed by 7-hydroxy-3,4-dihydrocadalin might be related to the anti-inflammatory activity on the topical oedema induced by croton oil.