Structure and Expression Analyses of a Gene Encoding Fructose-6-Phosphate, 2-Kinase/Fructose-2,6-Bisphosphatase from Maize

作     者：王东 杜喜玲 张红生 钱晓茵 杨金水 翟虎渠 

作者机构：南京农业大学作物遗传与种质创新国家重点实验室南京210095 复旦大学生命科学院遗传学研究所上海200433 

出 版 物：《Acta Botanica Sinica》 (Acta Botanica Sinica（植物学报：英文版）)

年 卷 期：2003年第45卷第4期

页      面：466-471页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：教育部博士点基金 ( 2 0 0 10 30 70 0 3) 

主　　题：maize fructose-2,6-bisphosphate fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase seed vigor 

摘      要：A full-length cDNA encoding fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase from maize (Zea mays L.) was cloned by the methods of reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE), and designated as mF2KP. The encoded protein is composed of two regions. Its COOH-terminal region is catalytic region and homologous to the enzymes from other eukaryotes; and its NH 2-terminal region is common and special region only in plant. A truncated fragment of mF2KP covering integrated catalytic region was expressed in Escherichia coli. The fusion protein had the activities of fructose-6-phosphate, 2-kinase as well as fructose-2,6-bisphosphatase. Northern blot showed that the transcript level of mF2KP in seedlings initiated from strong-vigor seeds is lower than that from weak-vigor seeds.