Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses

作     者：Hiono, Takahiro Matsuda, Atsushi Wagatsuma, Takanori Okamatsu, Masatoshi Sakoda, Yoshihiro Kuno, Atsushi 

作者机构：Natl Inst Adv Ind Sci & Technol Biotechnol Res Inst Drug Discovery Glycosci & Glycotechnol Res Grp Tsukuba Ibaraki 3058568 Japan Keio Univ Sch Med Dept Biochem Shinjuku Ku Tokyo 1608582 Japan Japan Bioind Assoc Project Utilizing Glycans Dev Innovat Drug Discov Chuo Ku Tokyo 1040032 Japan Hokkaido Univ Fac Vet Med Dept Dis Control Microbiol Lab Sapporo Hokkaido 0600818 Japan 

出 版 物：《VIROLOGY》 (病毒学)

年 卷 期：2019年第527卷

页      面：132-140页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：Japan Society for the Promotion of Science (JSPS) KAKENHI [16H06597, 18K15176] Grants-in-Aid for Scientific Research [16H06597, 18K15176] Funding Source: KAKEN 

主　　题：Influenza A virus Lectin microarray Hemagglutinin Glycosylation alpha-Gal 

摘      要：Glycan structures on hemagglutinin (HA) of influenza A viruses have been analyzed previously to understand their significance. However, the formerly established methods using mass spectrometry present disadvantages such as procedure complexity, sensitivity, and throughput. Our study has established a novel method for analyzing glycan profiles of HA using lectin microarray techniques. We successfully obtained glycan profiles of HA starting from 1 ml of the 10(6) TCID50 samples through simple antigen enrichment using optimized immunoprecipitation. The profiles were reasonably consistent with known glycan structures of HA. Next, we compared glycan profiles of the HAs prepared from chicken embryos, MDCK, Vero, and A549 cells, and demonstrated the host cell-specific HA glycan profiles. Notably, the HA from MDCK cells was alpha 1-3 galactosylated. Our method provides a highly sensitive and simple procedure for glycan profiling of the viral glycoproteins, thereby paving way for direct glycan analyses of human- and animal-derived virions.