Functional characterization of the keratinocyte growth factor system in human fetal gastrointestinal tract

作     者：Chailler, P Basque, JR Corriveau, L Ménard, D 

作者机构：Univ Sherbrooke Fac Med Dept Anat & Biol Cellulaire MRC Grp Funct Dev & Physiopathol Digest Tract Sherbrooke PQ J1H 5N4 Canada 

出 版 物：《PEDIATRIC RESEARCH》 (儿科研究)

年 卷 期：2000年第48卷第4期

页      面：504-510页

核心收录：

学科分类：1002[医学-临床医学] 100202[医学-儿科学] 10[医学] 

主　　题：印迹法 蛋白质 结肠/化学 结肠/胚胎学 结肠/酶学 培养技术 DNA/生物合成 消化系统/化学 消化系统/胚胎学 上皮细胞/化学 食管/化学 食管/胚胎学 成纤维细胞生长因子10 成纤维细胞生长因子7 成纤维细胞生长因子 荧光抗体技术 间接 孕龄 生长物质/分析 生长物质/药理学 小肠/化学 小肠/胚胎学 空肠/酶学 受体 成纤维细胞生长因子 2型 受体 成纤维细胞生长因子 受体 生长因子/分析 胃/化学 胃/胚胎学 蔗糖酶异麦芽糖酶复合物/代谢 γ-谷氨酰转移酶/代谢 女(雌)性 人类 妊娠 

摘      要：Keratinocyte growth factor (KGF) is a paracrine growth factor whose mRNA has been detected in human adult and rodent gut tissues together with its associated receptor. Our objectives were to assess the presence of immunoreactive KGF ligand and receptor proteins in human fetal gastrointestinal (GI) tract segments and to evaluate the role of exogenous KGF on cell proliferation and intestinal digestive functions. KGF (26-28 kD doublet) was identified in esophagus, stomach, small intestine, and colon by Western blot. Its receptor (135 kD) was ubiquitously detected in proliferative and differentiated epithelial cells of each GI segment by use of indirect immunofluorescence (anti-bek, anti-K-sam). The addition of KGF to explants cultured in serum-free conditions greatly stimulated DNA synthesis in all GI tract tissues. The growth factor up-regulated intestinal sucrase-isomaltase and gamma-glutamyl-transpeptidase activities in jejunal exp]ants, whereas it down-regulated these activities in colon explants. It is suggested that the KGF system likely represents an important paracrine pathway that is able to stimulate cell proliferation in all segments of the human fetal GI tract and to differentially regulate intestinal digestive functions.