Combined two-photon microscopy and optical coherence tomography using individually optimized sources

作     者：Jeong, Bosu Lee, Byunghak Jang, Min Seong Nam, Hyoseok Yoon, Sang June Wang, Taejun Doh, Junsang Yang, Bo-Gie Jang, Myoung Ho Kim, Ki Hean 

作者机构：Pohang Univ Sci & Technol Sch Interdisciplinary Biosci & Bioengn Pohang 790784 Gyeongbuk South Korea Pohang Univ Sci & Technol Dept Mech Engn Pohang 790784 Gyeongbuk South Korea Pohang Univ Sci & Technol Div Integrat Biosci & Biotechnol Pohang 790784 Gyeongbuk South Korea Osaka Univ Lab Gastrointestinal Immunol WPI Immunol Frontier Res Ctr Suita Osaka 5650871 Japan 

出 版 物：《OPTICS EXPRESS》 (Opt. Express)

年 卷 期：2011年第19卷第14期

页      面：13089-13096页

核心收录：

学科分类：070207[理学-光学] 07[理学] 08[工学] 0803[工学-光学工程] 0702[理学-物理学] 

基　　金：Korean Science Foundation [2010-0028014, 2010-0014874] Ministry of Education, Science and Technology [R31-2008-000-10105-0, R31-10105] 

主　　题：Adaptive optics Image processing algorithms In vivo imaging Nonlinear photonic crystals Photonic crystal fibers Second harmonic generation 

摘      要：The combination of two-photon microscopy (TPM) and optical coherence tomography (OCT) is useful in conducting in-vivo tissue studies, because they provide complementary information regarding tissues. In the present study, we developed a new combined system using separate light sources and scanners for individually optimal imaging conditions. TPM used a Ti-Sapphire laser and provided molecular and cellular information in microscopic tissue regions. Meanwhile, OCT used a wavelength-swept source centered at 1300 nm and provided structural information in larger tissue regions than TPM. The system was designed to do simultaneous imaging by combining light from both sources. TPM and OCT had the field of view values of 300 mu m and 800 mu m on one side respectively with a 20x objective. TPM had resolutions of 0.47 mu m and 2.5 mu m in the lateral and axial directions respectively, and an imaging speed of 40 frames/s. OCT had resolutions of 5 mu m and 8 mu m in lateral and axial directions respectively, a sensitivity of 97dB, and an imaging speed of 0.8 volumes per second. This combined system was tested with simple microsphere specimens, and was then applied to image small intestine and ear tissues of mouse models ex-vivo. Molecular, cellular, and structural information of the tissues were visualized using the proposed combined system. (C) 2011 Optical Society of America