Chemical Composition and Anti-inflammatory Properties of the Unsaponifiable Fraction from Awara (<i>Astrocaryum vulgare</i> M.) Pulp Oil in Activated J774 Macrophages and in a Mice Model of Endotoxic Shock

作     者：Bony, Emilie Boudard, Frederic Dussossoy, Emilie Portet, Karine Brat, Pierre Giaimis, Jean Michel, Alain 

作者机构：Univ Montpellier I Fac Pharm UMR Qualisud Lab Pharmacol & Physiopathol Expt F-34093 Montpellier 5 France Univ Montpellier I Fac Pharm Immunol Lab F-34093 Montpellier 5 France Ctr Cooperat Int Rech Agron Dev CIRAD Dept PERSYST UMR Qualisud F-34398 Montpellier 5 France 

出 版 物：《PLANT FOODS FOR HUMAN NUTRITION》 (人类营养植物食品)

年 卷 期：2012年第67卷第4期

页      面：384-392页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 071001[理学-植物学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 07[理学] 0703[理学-化学] 

主　　题：Awara (Astrocaryum vulgare M.) Unsaponifiable fraction Carotenoids Phytosterols Tocopherols Anti-inflammatory J774 macrophages Endotoxic shock 

摘      要：Awara (Astrocaryum vulgare M.) pulp oil has been shown to possess anti-inflammatory properties in vivo, and contains an unsaponifiable matter rich in bioactive compounds. This study focused on the ethanolic unsaponifiable fraction (EUF) of awara pulp oil. Its chemical composition has been characterized: carotenoid, phytosterol, and tocopherol contents represent 125.7, 152.6, and 6.8 mu g/mg of EUF, respectively. We further evaluated this fraction for anti-inflammatory properties in J774 macrophages activated by lipopolysaccharide (LPS) plus interferon (IFN) gamma to understand the biological effects of awara pulp oil. EUF strongly decreased nitric oxide (NO), prostaglandin E-2, tumour necrosis factor (TNF) alpha, and interleukin (IL) -6 and -10 production in activated J774 cells. Moreover, it inhibited expression of inducible NO synthase and cyclooxygenases-2 in vitro. The anti-inflammatory properties of EUF were also confirmed in vivo by modulation of TNF alpha, IL-6 and IL-10 serum concentration in an endotoxic shock model. Pre-treatment with awara oil fraction offers promise as a protective means to lower the production of excessive amounts of pro-inflammatory molecules.