Short-Range Spectroscopic Ruler Based on a Single-Molecule Optical Switch

作     者：Mark Bates Timothy R. Blosser Xiaowei Zhuang 

作者机构：Division of Engineering and Applied Sciences Harvard University Cambridge Massachusetts 02138 USA Graduate Program in Biophysics Harvard University Cambridge Massachusetts 02138 USA Department of Chemistry Harvard University Cambridge Massachusetts 02138 USA Department of Physics Harvard University Cambridge Massachusetts 02138 USA 

出 版 物：《Physical Review Letters》 (Phys Rev Lett)

年 卷 期：2005年第94卷第10期

页      面：108101-108101页

核心收录：

学科分类：07[理学] 0702[理学-物理学] 

基　　金：National Institute of General Medical Sciences, NIGMS, (R01GM068518) National Institute of General Medical Sciences, NIGMS 

主　　题：.switching distance PRL ending Probe dependence Demonstrate Spectroscopic uorescent short-range Single-molecule switched optical switch Ruler dark state 

摘      要：We demonstrate a novel all-optical switch consisting of two molecules: a primary fluorophore (Cy5) that can be switched between a fluorescent and a dark state by light of different wavelengths, and a secondary chromophore (Cy3) that facilitates switching. The interaction between the two molecules exhibits a distance dependence much steeper than that of conventional Förster resonance energy transfer. This enables the switch to act as a ruler with the capability to probe distances difficult to access by other spectroscopic methods, thus presenting a new tool for the study of biomolecules at the single-molecule level.