Unzipping Kinetics of Double-Stranded DNA in a Nanopore

作     者：Alexis F. Sauer-Budge Jacqueline A. Nyamwanda David K. Lubensky Daniel Branton 

作者机构：Biophysics Program Harvard University Cambridge Massachusetts 02138 USA Department of Molecular & Cellular Biology Harvard University Cambridge Massachusetts 02138 USA Bell Labs Lucent Technologies 700 Mountain Avenue Murray Hill New Jersey 07974 USA 

出 版 物：《Physical Review Letters》 (Phys Rev Lett)

年 卷 期：2003年第90卷第23期

页      面：238101-238101页

核心收录：

学科分类：07[理学] 0702[理学-物理学] 

基　　金：U.S. Department of Energy, USDOE Defense Advanced Research Projects Agency, DARPA 

主　　题：.dsDNA strands enthalpy nanopore double-stranded strand separation Unzipping effective charge protein pore 

摘      要：We studied the unzipping of single molecules of double-stranded DNA by pulling one of their two strands through a narrow protein pore. Polymerase chain reaction analysis yielded the first direct proof of DNA unzipping in such a system. The time to unzip each molecule was inferred from the ionic current signature of DNA traversal. The distribution of times to unzip under various experimental conditions fit a simple kinetic model. Using this model, we estimated the enthalpy barriers to unzipping and the effective charge of a nucleotide in the pore, which was considerably smaller than previously assumed.