Genome-wide identification and characterization of myosin genes in the silkworm, Bombyx mori

作     者：Tan, Duan Hu, Hai Tong, Xiaoling Han, Minjin Zuo, Weidong Dai, Fangyin Lu, Cheng 

作者机构：Southwest Univ State Key Lab Silkworm Genome Biol Key Lab Sericultural Biol & Genet Breeding Minist AgrColl Biotechnol Chongqing 400715 Peoples R China 

出 版 物：《GENE》 (基因)

年 卷 期：2019年第691卷

页      面：45-55页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：Hi-Tech Research and Development 863 Program of China [2013AA102507] National Natural Science Foundation of China Chongqing Postdoctoral Science Special Foundation [Xm2017146] Fund for China Agriculture Research System [CARS-18] Fundamental Research Funds for the Central Universities [XDJK2017C042] 

主　　题：Myosin Unconventional myosin Phylogenetic analysis Expression profiles Bombyx mori 

摘      要：Myosins are a large family of actin filament-based motor proteins with a broad range of functions such as intracellular membrane trafficking, endocytosis, exocytosis, organellar transport, growth cone motility, cytokinesis, and cell locomotion. They are found in many organisms from fungi to humans. The myosin gene family in Bombyx mori is poorly studied, even though the molecular functions of these genes in vertebrates and insects, such as Drosophila, are well known. We identified 16 myosin genes from B. mori and identified the myosin genes in 12 vertebrates, eight insects, three nematodes, and seven protozoa. The number of myosin genes in vertebrates is double the number in invertebrates. The number of myosin isoforms in classes I and II is larger in vertebrates compared to invertebrates. B. mori myosin genes can be classified into 11 classes. Compared to B. mori, some myosin classes are not present in other insects. Classes I, II, XVIII, and XXI appear to be important for insect survival because they are conserved among nine insects. The relatively large sizes of B. mori myosin genes are due to their longer introns. Reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) analysis demonstrated that many B. mori myosin genes have tissue-specific expression and exhibit temporal specific activity during metamorphosis. These data provide insights into evolutionary and functional aspects of B. mori myosin genes that could be useful for the study of homologous myosins in other Lepidoptera species.