The Yucatan Minipig Temporomandibular Joint Disc Structure-Function Relationships Support Its Suitability for Human Comparative Studies

作     者：Vapniarsky, Natalia Aryaei, Ashkan Arzi, Boaz Hatcher, David C. Hu, Jerry C. Athanasiou, Kyriacos A. 

作者机构：Univ Calif Davis Dept Biomed Engn Davis CA 95616 USA Univ Calif Davis Dept Surg & Radiol Sci Sch Vet Med Davis CA 95616 USA Diagnost Digital Imaging Ctr Sacramento CA USA Univ Calif Davis Dept Orthoped Surg Sch Med Davis CA 95616 USA 

出 版 物：《TISSUE ENGINEERING PART C-METHODS》 (Tissue Eng. Part. C Methods)

年 卷 期：2017年第23卷第11期

页      面：700-709页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 0805[工学-材料科学与工程（可授工学、理学学位）] 10[医学] 

基　　金：NIH [R01DE015038] National Institute of Dental and Craniofacial Research [R01DE015038] Funding Source: NIH RePORTER 

主　　题：temporomandibular joint disc characterization structure-function large animal model translation anisotropy 

摘      要：Frequent involvement of the disc in temporomandibular joint (TMJ) disorders warrants attempts to tissue engineer TMJ disc replacements. Physiologically, a great degree of similarity is seen between humans and farm pigs (FPs), but the pig s rapid growth confers a significant challenge for in vivo experiments. Minipigs have a slower growth rate and are smaller than FPs, but minipig TMJ discs have yet to be fully characterized. The objective of this study was to determine the suitability of the minipig for TMJ studies by extensive structural and functional characterization. The properties of minipig TMJ discs closely reproduced previously reported morphological, biochemical, and biomechanical values of human and FP discs. The width/length dimension ratio of the minipig TMJ disc was 1.95 (1.69 for human and 1.94 for FP). The biochemical evaluation revealed, on average per wet weight, 24.3% collagen (22.8% for human and 24.9% for FP);0.8% glycosaminoglycan (GAG;0.5% for human and 0.4% for FP);and 0.03% DNA (0.008% for human and 0.02% for FP). Biomechanical testing revealed, on average, compressive relaxation modulus of 50kPa (37kPa for human and 32kPa for FP), compressive instantaneous modulus of 1121kPa (1315kPa for human and 1134kPa for FP), and coefficient of viscosity of 13MPas (9MPas for human and 3MPas for FP) at 20% strain. These properties also varied topographically in accordance to those of human and FP TMJ discs. Anisotropy, quantified by bidirectional tensile testing and histology, again was analogous among minipig, human, and FP TMJ discs. The minipig TMJ s ginglymoarthrodial nature was verified through cone beam computer tomography. Collectively, the similarities between minipig and human TMJ discs support the use of minipig as a relevant model for TMJ research;considering the practical advantages conferred by its growth rate and size, the minipig may be a preferred model over FP.