Cell-Surface Expression of Neuron-Glial Antigen 2 (NG2) and Melanoma Cell Adhesion Molecule (CD146) in Heterogeneous Cultures of Marrow-Derived Mesenchymal Stem Cells

作     者：Russell, Katie C. Tucker, H. Alan Bunnell, Bruce A. Andreeff, Michael Schober, Wendy Gaynor, Andrew S. Strickler, Karen L. Lin, Shuwen Lacey, Michelle R. O'Connor, Kim C. 

作者机构：Tulane Univ Dept Chem & Biomol Engn New Orleans LA 70118 USA Tulane Univ Sch Med Ctr Stem Cell Res & Regenerat Med New Orleans LA 70118 USA Tulane Univ Sch Med Biomed Sci Grad Program New Orleans LA 70118 USA Univ Texas MD Anderson Canc Ctr Dept Leukemia Houston TX 77030 USA Tulane Univ Dept Math New Orleans LA 70118 USA 

出 版 物：《TISSUE ENGINEERING PART A》 (Tissue Eng. Part A)

年 卷 期：2013年第19卷第19-20期

页      面：2253-2266页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 0805[工学-材料科学与工程（可授工学、理学学位）] 10[医学] 

基　　金：National Science Foundation [CBET-1066167] IDEA award from Tulane University National Institutes of Health [CA-55164, CA-16672, CA-100632, CA-136411] Directorate For Engineering Div Of Chem, Bioeng, Env, & Transp Sys Funding Source: National Science Foundation 

主　　题：Mesenchymal stem cell 

摘      要：Cellular heterogeneity of mesenchymal stem cells (MSCs) impedes their use in regenerative medicine. The objective of this research is to identify potential biomarkers for the enrichment of progenitors from heterogeneous MSC cultures. To this end, the present study examines variation in expression of neuron-glial antigen 2 (NG2) and melanoma cell adhesion molecule (CD146) on the surface of MSCs derived from human bone marrow in response to culture conditions and among cell populations. Multipotent cells isolated from heterogeneous MSC cultures exhibit a greater than three-fold increase in surface expression for NG2 and greater than two-fold increase for CD146 as compared with parental and lineage-committed MSCs. For both antigens, surface expression is downregulated by greater than or equal to six-fold when MSCs become confluent. During serial passage, maximum surface expression of NG2 and CD146 is associated with minimum doubling time. Upregulation of NG2 and CD146 during loss of adipogenic potential at early passage suggests some limits to their utility as potency markers. A potential relationship between proliferation and antigen expression was explored by sorting heterogeneous MSCs into rapidly and slowly dividing groups. Fluorescence-activated cell sorting revealed that rapidly dividing MSCs display lower scatter and 50% higher NG2 surface expression than slowly dividing cells, but CD146 expression is comparable in both groups. Heterogeneous MSCs were sorted based on scatter properties and surface expression of NG2 and CD146 into high (HI) and low (LO) groups. Sc(LO)NG2(HI) and Sc(LO)NG2(HI)CD146(HI) MSCs have the highest proliferative potential of the sorted groups, with colony-forming efficiencies that are 1.5-2.2 times the value for the parental controls. The Sc-LO gate enriches for rapidly dividing cells. Addition of the NG2(HI) gate increases cell survival to 1.5 times the parental control. Further addition of the CD146(HI) gate does not significantly impro