Simultaneous HPLC determination of alfuzosin, tamsulosin and vardenafil in human plasma and pharmaceutical formulations using time programmed fluorescence detection

作     者：Walash, M. I. Belal, F. Fathy, M. Zayed, S. Borg, H. 

作者机构：Univ Mansoura Dept Pharmaceut Analyt Chem Fac Pharm Mansoura 35516 Egypt Univ Mansoura Fac Pharm Unit Drug Anal Mansoura 35516 Egypt Delta Univ Sci & Technol Fac Pharm Dept Pharmaceut Chem Int Coastal Rd Gamasa City Dakahliya Egypt 

出 版 物：《ANNALES PHARMACEUTIQUES FRANCAISES》 (法国药学纪事)

年 卷 期：2019年第77卷第1期

页      面：28-37页

学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学] 

主　　题：Fluorescence detection Time programming Human plasma Alfuzosin Tamsulosin Vardenafil 

摘      要：Alfuzosin and tamsulosin are recently co-administrated with vardenafil to treat symptoms of benign prostatic hyperplasia and erectile dysfunction. A highly sensitive and simple liquid chromatographic method was developed and validated for the simultaneous determination of the three drugs using moxifloxacin as an internal standard. lsocratic separation was achieved within 7.0 min using phenyl-hexyl column (250 :, 4.6 mm i.d.) and a mobile phase composed of acetonitrile/0.25% phosphoric acid (30:70, v/v) at pH 3.0. The analysis was performed at a flow rate of 1.2 mL/min with fluorescence detection at 246/450 nm for Alfuzosin and vardenafil, and 226/322 nm for tamsulosin using time programming technique. The proposed method was linear over the concentration ranges of 5.0-50.0 ng/mL, 10.0-200.0 ng/mL and 20.0-400.0 ng/mL for alfuzosin, vardenafil and tamsulosin, with limits of detection of 0.56 ng/mL, 0.98 ng/mL and 2.81 ng/mL in a respective order. The developed method was successfully applied to determine the studied drugs in dosage forms and human plasma samples and the results were satisfactory as revealed by statistical analysis of the data. (C) 2018 Academie Nationale de Pharmacie. Published by Elsevier Masson SAS. All rights reserved.