Advanced Substrate Fabrication for Cell Microarrays

作     者：Hook, Andrew L. Thissen, Helmut Voelcker, Nicolas H. 

作者机构：Flinders Univ S Australia Sch Chem Phys & Earth Sci Bedford Pk SA 5042 Australia CSIRO Mol & Hlth Technol Clayton Vic 3168 Australia 

出 版 物：《BIOMACROMOLECULES》 (生物大分子)

年 卷 期：2009年第10卷第3期

页      面：573-579页

核心收录：

学科分类：0710[理学-生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：Flinders University Australian Research Council CSIRO 

主　　题：Azides/chemistry Cell Proliferation Coated Materials, Biocompatible/chemical synthesis Coated Materials, Biocompatible/chemistry Glass/chemistry Humans Macromolecular Substances/chemical synthesis Macromolecular Substances/chemistry Particle Size Polyethylene Glycols/chemistry Surface Properties Tissue Array Analysis/methods Tumor Cells, Cultured Ultraviolet Rays Ultraviolet Rays 

摘      要：The fabrication and characterization of chemical patterns using a technique that can be readily integrated with methods currently used for the formation of microarrays is presented. A high density poly(ethylene glycol) coating was deposited on glass slides as a background exhibiting low cell attachment properties. Phenylazide modified polymers were then printed on this background. UV irradiation of these polymer arrays resulted in the cross-linking of the polymer spots and their covalent attachment to the surface. Cell attachment was shown to follow the resultant surface chemistry pattern. Furthermore, the use of a robotic contact printer enabled the facile deposition of DNA microarrays on top of and aligned with the polymer microarrays. A transfected cell microarray was generated in this way, demonstrating not only the ability of this platform to limit cell attachment to specific regions, but the suitability for chip-based functional genomics, in particular, and high density cell assays in general.