<i>In vitro</i> ischemia-reperfusion injury in term human placenta as a model for oxidative stress in pathological pregnancies

作     者：Hung, TH Skepper, JN Burton, GJ 

作者机构：Univ Cambridge Dept Anat Cambridge CB2 3DY England Chang Gung Mem Hosp Dept Obstet & Gynaecol Taipei 10591 Taiwan 

出 版 物：《AMERICAN JOURNAL OF PATHOLOGY》 (美国病理学杂志)

年 卷 期：2001年第159卷第3期

页      面：1031-1043页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：Wellcome Trust Funding Source: Medline 

主　　题：醛类/代谢 缺氧/代谢 环氮氧化物 去铁胺/药理学 荧光抗体技术 HSP72热休克蛋白质类 热休克蛋白质类/代谢 免疫组织化学 缺血/代谢 氧化氮类/药理学 氧化性应激 氧/治疗应用 胎盘/血液供给 妊娠并发症/代谢 活性氧/代谢 再灌注损伤/代谢 超氧化物歧化酶/药理学 组织分布 酪氨酸/类似物和衍生物 酪氨酸/代谢 女(雌)性 人类 妊娠 

摘      要：Oxidative stress is a prominent feature of the placenta in many complications of pregnancy, such as preeclampsia. The cause is primarily unknown, although ischemia-reperfusion injury is one possible mechanism. Our aim was to test this hypothesis by examining the oxidative status of human placental tissues during periods of hypoxia and reoxygenation in vitro. Rapid generation of reactive oxygen species was detected using the fluorogenic probe, 2 ,7 -dichlorofluorescein diacetate, when hypoxic tissues were reoxygenated. The principal sites were the villous endothelium, and to a lesser extent the syncytiotrophoblast and stromal cells. Increased concentrations of heat shock protein 72, nitrotyrosine residues, and 4-hydroxy-2-nonenal were also observed in the villous endothelial and underlying smooth muscle cells, and in the syncytiotrophoblast. Furthermore, preloading placental tissues with the reactive oxygen species scavengers desferrioxamine and alpha -phenyl-N-tert-butylnitrone reduced levels of oxidative stress after reoxygenation. These changes are consistent with an ischemia-reperfusion injury, and mirror those seen in preeclampsia. Consequently, in vitro hypoxia/reoxygenation may represent a suitable model system for investigating the generation of placental oxidative stress in preeclampsia and other complications of pregnancy.