Cytosolic delivery of granzyme B by bacterial toxins: Evidence that endosomal disruption, in addition to transmembrane pore formation, is an important function of perforin

作     者：Browne, KA Blink, E Sutton, VR Froelich, CJ Jans, DA Trapani, JA 

作者机构：Austin Res Inst Heidelberg Vic 3084 Australia Australian Natl Univ John Curtin Sch Med Res Nucl Signalling Lab Canberra ACT 2600 Australia ENH Res Inst Cell Death Program Evanston IL 60201 USA 

出 版 物：《MOLECULAR AND CELLULAR BIOLOGY》 (分子生物学与细胞生物学)

年 卷 期：1999年第19卷第12期

页      面：8604-8615页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

主　　题：细胞凋亡 细菌蛋白质类 细菌毒素类/代谢 细菌毒素类/药理学 细胞核 胞质溶胶 内颗粒/代谢 颗粒酶类 热休克蛋白质类/代谢 热休克蛋白质类/药理学 溶血素蛋白质类/代谢 溶血素蛋白质类/药理学 Jurkat细胞 膜糖蛋白类/生理学 穿孔素 成孔毒素蛋白质类 丝氨酸内肽酶类/代谢 链球菌溶血素类/代谢 链球菌溶血素类/药理学 肿瘤细胞 培养的 动物 人类 小鼠 

摘      要：Granule-mediated cell killing by cytotoxic lymphocytes requires the combined actions of a membranolytic protein, perforin, and granule-associated granzymes, but the mechanism by which they jointly kill cells is poorly understood. me have tested a series of membrane-disruptive agents including bacterial pore-farming toxins and hemolytic complement for their ability to replace perforin in facilitating granzyme B-mediated cell death. As with perforin, low concentrations of streptolysin O and pneumolysin (causing 4,000 U/mI were perforin pores demonstrably large enough to account for transmembrane diffusion of granzyme B. We conclude that pore