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Migration behavior of isoluminol isothiocyanate-labeled α-amino acids in capillary electrophoresis with an absorption/chemiluminescence dual detection system

isoluminol 的迁居行为在有吸收 / 化合光的毛状的电气泳动的标记 isothiocyanate 的伪 - 氨基酸双检测系统

作     者:Tsukagoshi, Kazuhiko Sawanoi, Kaori Nakajima, Rfichiro 

作者机构:Doshisha Univ Fac Engn Dept Chem Engn & Mat Sci Kyotanabe 6100321 Japan 

出 版 物:《JOURNAL OF CHROMATOGRAPHY A》 (色谱法杂志A辑)

年 卷 期:2007年第1143卷第1-2期

页      面:288-290页

核心收录:

学科分类:0710[理学-生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

基  金:Ministry of Education  Culture  Sports  Science and Technology  MEXT 

主  题:capillary electrophoresis chemiluminescence absorption/chemiluminescence dual detection alpha-amino acids 

摘      要:In our previous study, we developed capillary electrophoresis with an ultraviolet absorption/chemiluminescence dual detection system. Here, to demonstrate one of the possible applications of the capable system as well as confirm the advantage, migration behavior of isoluminol isothiocyanate-labeled alpha-amino acids was examined in the capillary electrophoresis with a dual detection system. The labeled samples were first analyzed by absorption detection with an on-capillary, followed by chemilunminescence detection with an end-capillary. The system easily, rapidly, and simultaneously produced useful information concerning chemiluminescence quenching and amino group-labeling due to the presence of both absorption and chemiluminescence detections. (c) 2007 Elsevier B.V. All rights reserved.

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