The T-superfamily of conotoxins. (vol 274, pg 30664, 1999)

作     者：Walker, CS Steel, D Jacobsen, RB Lirazan, MB Cruz, LJ Hooper, D Shetty, R DelaCruz, RC Nielsen, JS Zhou, LM Bandyopadhyay, P Craig, AG Olivera, BM 

作者机构：DepartmentofBiologyUniversityofUtahSaltLakeCityUtah84112USA. 

出 版 物：《JOURNAL OF BIOLOGICAL CHEMISTRY》 (生物化学杂志)

年 卷 期：1999年第274卷第50期

页      面：36030-36030页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：National Institute of General Medical Sciences  NIGMS  (P01GM048677) 

主　　题：氨基酸序列 碱基序列 克隆 分子 芋螺毒素类/化学 芋螺毒素类/遗传学 芋螺毒素类/毒性 共有序列 半胱氨酸 鱼类 分子序列数据 运动活动/药物作用 蛋白质加工 转译后 蛋白分选信号/化学 重组蛋白质类/化学 序列比对 序列同源性 氨基酸 螺/遗传学 动物 小鼠 

摘      要：We report the discovery and initial characterization of the T-superfamily of conotoxins. Eight different T-superfamily peptides from five Conus species were identified; they share a consensus signal sequence, and a conserved arrangement of cysteine residues (- -CC- -CC-). T-superfamily peptides were found expressed in venom ducts of all major feeding types of Conus; the results suggest that the T-superfamily will be a large and diverse group of peptides, widely distributed in the 500 different Conus species. These peptides are likely to be functionally diverse; although the peptides are small (11-17 amino acids), their sequences are strikingly divergent, with different peptides of the superfamily exhibiting varying extents of post-translational modification. Of the three peptides tested for in vivo biological activity, only one was active on mice but all three had effects on fish. The peptides that have been extensively characterized are as follows: p5a, GCCPKQMRCCTL*; tx5a, γCCγDGW+CCT§AAO; and au5a, FCCPFIRYCCW (where γ = γ-carboxyglutamate, W^+ = bromotryptophan, O = hydroxyproline, T§ = glycosylated threonine, and * = COOH-terminal amidation). We also demonstrate that the precursor of tx5a contains a functional γ-carboxylation recognition signal in the -1 to -20 propeptide region, consistent with the presence of γ-carboxyglutamate residues in this peptide.