Effect of killer toxin K1 on yeast membrane potential reported by the diS-C₃(3) probe reflects strain- and physiological state-dependent variations

作     者：Eminger, M Gásková, D Brodská, B Holoubek, A Stadler, N Sigler, K 

作者机构：Charles Univ Inst Phys CR-12116 Prague Czech Republic Acad Sci Czech Republ Inst Microbiol CR-14220 Prague Czech Republic Univ Bonn Inst Bot D-53115 Bonn Germany 

出 版 物：《FOLIA MICROBIOLOGICA》 (微生物学报)

年 卷 期：1999年第44卷第3期

页      面：283-288页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 0836[工学-生物工程] 071005[理学-微生物学] 10[医学] 

基　　金：Grant Agency of the Czech Repttblic, (143/96, 204/95/1051, 204/96/1261) Grantová Agentura, Univerzita Karlova, GA, UK, (CRG.CRGP 973150) 

主　　题：羰花青/代谢 荧光染料/代谢 真菌蛋白质类/药理学 杀伤因子 酵母 膜电位/药物作用 真菌毒素类/药理学 制霉菌素/药理学 酿酒酵母菌/生理学 

摘      要：The rate and extent of uptake of the fluorescent probe diS-C-3(3) reporting on membrane potential in S. cerevisiae is affected by the strain under study, cell-growth phase, starvation and by the concentration of glucose both in the growth medium and in the monitored cell suspension under non-growth conditions. Killer toxin K1 brings about changes in membrane potential. In all types of cells tested, viz. in glucose-supplied stationary or exponential cells of the killer-sensitive strain S6/1 or a conventional strain RXII, or in glucose-free exponential cells of both strains, both active and heat-inactivated toxin slow down the potential-dependent uptake of diS-C-3(3) into the cells. This may reflect clogging of pores in the cell wall that hinders, but does not prevent, probe passage to the plasma membrane and its equilibration. The clogging effect of heat-inactivated toxin is stronger than that exerted by active toxin. In susceptible cells, ie. in exponential-phase glucose-supplied cells of the sensitive strain S6/1, this phase of probe uptake retardation is followed by an irreversible red shift in probe fluorescence maximum lambda(max) indicating damage to membrane integrity and cell permeabilization. A similar fast red shift in IZ,, signifying lethal cell damage was found in heat-killed or nystatin-treated cells.