Lipoprotein lipase enhances the binding of native and oxidized low density lipoproteins to versican and biglycan synthesized by cultured arterial smooth muscle cells

作     者：Olin, KL Potter-Perigo, S Barrett, PHR Wight, TN Chait, A 

作者机构：Univ Washington Dept Med Seattle WA 98195 USA Univ Washington Dept Pathol Seattle WA 98195 USA Univ Western Australia Dept Med Perth WA 6001 Australia 

出 版 物：《JOURNAL OF BIOLOGICAL CHEMISTRY》 (生物化学杂志)

年 卷 期：1999年第274卷第49期

页      面：34629-34636页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：NHLBI NIH HHS [HL07028] Funding Source: Medline NIDDK NIH HHS [DK02456, DK07247] Funding Source: Medline 

主　　题：动脉/代谢 双糖链蛋白多糖 细胞 培养的 硫酸软骨素蛋白聚糖类/代谢 剂量效应关系 药物 细胞外基质蛋白质类 动力学 外源凝集素类 C型 脂蛋白脂酶/药理学 脂蛋白类 LDL/代谢 乳蛋白质类/代谢 肌 平滑 血管/代谢 氧化还原 蛋白质结合/药物作用 蛋白聚糖类/代谢 多功能蛋白聚糖类 动物 牛 人类 

摘      要：Retention of low density lipoproteins (LDL) by vascular proteoglycans and their subsequent oxidation are important in atherogenesis, Lipoprotein lipase (LPL) can bind LDL and proteoglycans, although the effect of different proteoglycans to influence the ability of LPL to act as a bridge in the formation of LDL-proteoglycan complexes is unknown. Using an electrophoretic gel mobility shift assay, [S-35]SO4-labeled versican and biglycan, two extracellular proteoglycans secreted by vascular cells, bound native LDL in a saturable fashion, The addition of bovine milk LPL dose-dependently increased the binding of native LDL to both versican and biglycan, approaching saturation at 30-40 mu g/ml LPL for versican and 20 mu g/ml LPL for biglycan. LDL was oxidized by several methods, including copper, 2,2-azobis(2-amidinopropane)-2HCl and hypochlorite, Extensively copper- and hypochlorite-oxidized LDL bound poorly to versican and biglycan, Proteoglycan binding to LDL was correlated inversely with the extent of LDL;however, the addition of LPL to oxidized LDL together with biglycan or versican allowed the oxidized LDL to bind the proteoglycans in an LPL dose-dependent manner. Addition of LPL had a greater relative effect on the binding of extensively oxidized LDL to proteoglycans compared with native LDL, LPL had a slightly greater effect on increasing the binding of native and oxidized LDL to biglycan than versican, Thus, LPL in the artery wall might increase the atherogenicity of oxidized LDL, since it enables its binding to vascular biglycan and versican.