A validation study of a multiplex INDEL assay for forensic use in four Chinese populations

作     者：Yi-Liang Wei Cui-Jiao Qin Hui Dong Jing Jia Cai-Xia Li 

作者机构：Key Laboratory of Forensic Genetics Institute of Forensic Science Ministry of Public Security Beijing 100038 PR China Key Laboratory of Medical Epigenetics Tianjin Research Center of Basic Medical Sciences Tianjin 300070 PR China Department of Forensic Science Chinese People's Public Security University Beijing 100038 PR China Department of Forensic Science Southern Medical University Guangzhou 510515 PR China Faculty of Basic Medical Sciences Chongqing Medical University Chongqing 400016 PR China 

出 版 物：《Forensic Science International: Genetics》 

年 卷 期：2014年第9卷

页      面：e22-e25页

学科分类：1002[医学-临床医学] 1009[医学-特种医学] 10[医学] 

主　　题：INDEL Individual identification Population genetics Heterozygosity F st 

摘      要：Insertion/deletion (INDELs) marker sets can serve as a useful supplementary tool for human identification. A commercial kit, the Qiagen DIPplex ® Investigator kit, multiplexes 30 biallelic autosomal INDELs and Amelogenin for forensic use. We performed a validation study based on the DIPplex ® kit in four Chinese populations: Han, Tibetan, Uyghur, and Kazakh. There were no significant departures from Hardy–Weinberg equilibrium or significant linkage disequilibrium (pair-wised r 2 0.99. The multiplex PCR was optimized for a 5-μL volume, full profiles were obtained with 0.062 ng/μL of template DNA, and excellent performance was obtained with degraded casework samples. This study demonstrates that the multiplex INDEL assay can be used as a supplementary method for degraded DNA detection in the studied Chinese populations.