Sequence and initial characterization of the U_L10 (glycoprotein M) and U_L11 homologous genes of serotype 1 Marek's Disease Virus

作     者：Osterrieder, N 

作者机构：Fed Res Ctr Virus Dis Anim Friedrich Loeffler Inst Inst Mol Biol D-17498 Insel Reims Germany 

出 版 物：《ARCHIVES OF VIROLOGY》 (病毒学文献)

年 卷 期：1999年第144卷第9期

页      面：1853-1863页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

主　　题：氨基酸序列 细胞系 鸭 糖蛋白类/化学 糖蛋白类/遗传学 糖蛋白类/代谢 疱疹病毒2型 鹑鸡/化学 疱疹病毒2型 鹑鸡/遗传学 疱疹病毒2型 鹑鸡/生理学 分子序列数据 转录 遗传 病毒蛋白质类/化学 病毒蛋白质类/遗传学 病毒蛋白质类/代谢 病毒结构蛋白质类/化学 病毒结构蛋白质类/遗传学 病毒结构蛋白质类/代谢 动物 

摘      要：The nucleotide sequence of the U-L 10 (glycoprotein M) and the U-L 11 homologs of Marek s Disease Virus 1 strain GA was determined. The UL10 open reading frame encodes a type III membrane protein of 424 amino acids that contains eight hydrophobic domains and two consensus N-linked glycosylation sites. The U(L)11 homologous gene encodes an 84 amino acid polypeptide, and contains a highly conserved myristylation site at its aminoterminus. By analysis of infected-cell RNA with strand-specific RNA probes, transcription of both U(L)10 and U-L 11 in infected cells was demonstrated. Coupled in vitro transcription-translation confirmed that the U-L 10 product is a 47 kD N-glycosylated viral protein that aggregated upon boiling, whereas the U-L 11 protein exhibited a size of 12 kD after in vitro translation.