Subcellular localization of hepatitis C viral proteins in mammalian cells

作     者：Kim, JE Song, WK Chung, KM Back, SH Jang, SK 

作者机构：Pohang Univ Sci & Technol Dept Life Sci Pohang 790784 South Korea Kwangju Inst Sci Technol Dept Life Sci Kwangju South Korea 

出 版 物：《ARCHIVES OF VIROLOGY》 (病毒学文献)

年 卷 期：1999年第144卷第2期

页      面：329-343页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

主　　题：CHO细胞 COS细胞 细胞核/代谢 仓鼠亚科 细胞质/代谢 基因表达 绿色荧光蛋白质类 肝炎病毒属/遗传学 肝炎病毒属/代谢 发光蛋白质类/分析 发光蛋白质类/遗传学 显微镜检查 荧光 重组融合蛋白质类/分析 重组融合蛋白质类/遗传学 病毒非结构蛋白质类/分析 病毒非结构蛋白质类/遗传学 病毒蛋白质类/分析 病毒蛋白质类/遗传学 动物 

摘      要：We determined the subcellular localization of hepatitis C viral (HCV) proteins as a first step towards the understanding of the functions of these proteins in the mammalian cell (CHO-KI). We used fluorescence emitted from green fluorescent protein (GFP)-fused to the viral proteins to determine the subcellular localization of the viral proteins. We found that most of the viral proteins were excluded from the nucleus. Core exhibited a globular pattern near the nucleus. NS2 was concentrated in the perinuclear space. NS4A accumulated in the ER and the Golgi regions. NS3 was detected in the nucleus as well as the cytoplasm, when it was expressed by itself. However, NS3 became restricted to the cytoplasm, when it was produced together with NS4A. NS4B showed a spot-like pattern throughout the cytoplasm. NS5A and NS5B were distributed throughout the cytoplasm in a mesh-like pattern. These results can provide a basis for further investigations into the functions of the HCV proteins.