Efficacy of electrolyzed oxidizing water for inactivating <i>Escherichia coli</i> O157:H7, <i>Salmonella enteritidis</i>, and <i>Listeria monocytogenes</i>

作     者：Venkitanarayanan, KS Ezeike, GO Hung, YC Doyle, MP 

作者机构：Univ Georgia Coll Agr & Environm Sci Ctr Food Safety & Qual Enhancement Griffin GA 30223 USA Univ Connecticut Dept Anim Sci Storrs CT 06269 USA 

出 版 物：《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 (应用与环境微生物学)

年 卷 期：1999年第65卷第9期

页      面：4276-4279页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 0836[工学-生物工程] 071005[理学-微生物学] 10[医学] 

主　　题：集落计数 微生物 电解 大肠杆菌O157/生长和发育 利斯特菌 单核细胞增生/生长和发育 氧化还原 沙门菌 肠炎/生长和发育 温度 水 水微生物学 

摘      要：The efficacy of electrolyzed oxidizing water for inactivating Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes was evaluated. A five-strain mixture. of E. coli O157:H7, S, enteritidis, or L. monocytogenes of approximately 10(8) CFU/ml was inoculated in 9 ml of electrolyzed oxidizing water (treatment) or 9 mi of sterile, deionized water (control) and incubated at 4 or 23 degrees C for 0, 5, 10, and 15 min;at 35 degrees C for 0, 2, 4, and 6 min;or at 45 degrees C for 0, 1, 3, and 5 min. The surviving population of each pathogen at each sampling time was determined on tryptic soy agar. At 4 or 23 degrees C, an exposure time of 5 min reduced the populations of all three pathogens in the treatment samples by approximately 7 log CFU/ml, with complete inactivation by 10 min of exposure. A reduction of greater than or equal to 7 log CFU/ml in the levels of the three pathogens occurred in the treatment samples incubated for 1 min at 45 degrees C or for 2 min at 35 degrees C. The bacterial counts of all three pathogens in control samples remained the same throughout the incubation at all four temperatures. Results indicate that electrolyzed oxidizing water may be a useful disinfectant, but appropriate, applications need to be validated.