Genetic control of telomere integrity in <i>Schizosaccharomyces pombe</i>:: <i>rad3<SUP>+</SUP></i> and <i>tel1<SUP>+</SUP></i> are parts of two regulatory networks independent of the downstream protein kinases <i>chk1<SUP>+</SUP></i> and <i>cds1<SUP>+</SUP></i>

作     者：Matsuura, A Naito, T Ishikawa, F 

作者机构：Tokyo Inst Technol Grad Sch Biosci & Biotechnol Dept Life Sci Midori Ku Yokohama Kanagawa 2268501 Japan 

出 版 物：《GENETICS》 (遗传学)

年 卷 期：1999年第152卷第4期

页      面：1501-1512页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

主　　题：腺苷三磷酸酶类/遗传学 腺苷三磷酸酶类/生理学 细胞周期蛋白质类 染色体 真菌/超微结构 DNA螺旋酶类/遗传学 DNA螺旋酶类/生理学 DNA结合蛋白质类 真菌蛋白质类/遗传学 真菌蛋白质类/生理学 乳清酸/类似物和衍生物 乳清酸/药理学 蛋白激酶类/生理学 蛋白质丝氨酸苏氨酸激酶 蛋白质类/生理学 辐射耐受性/遗传学 酿酒酵母蛋白质类 裂殖酵母菌属/药物作用 裂殖酵母菌属/遗传学 裂殖酵母菌属/超微结构 非洲粟酒人体酵母蛋白质类 信号传导/生理学 端粒/超微结构 肿瘤抑制蛋白质类 

摘      要：The Schizosaccharomyces pombe checkpoint gene named rad3(+) encodes an ATM-homologous protein kinase that shares a highly conserved motif with proteins involved in DNA metabolism. Previous studies have shown that Rad3 fulfills its function via the regulation of the Chk1 and Cds1 protein kinases. Here we describe a novel role for Rad3 in the control of telomere integrity. Mutations in the rad3(+) gene alleviated telomeric silencing and produced shortened lengths in the telomere repeat tracts. Genetic analysis revealed that the other checkpoint rad mutations rad1, rad17, and rad26 belong to the same phenotypic class with rad3 with regard to control of the telomere length. Of these mutations, rad3 and rad26 have a drastic effect on telomere shortening, tel1(+), another ATM homologue in S. pombe, carries out its telomere maintenance function in parallel with the checkpoint rad genes. Furthermore, either a single or double disruption of cds1(+) and chk1(+) caused no obvious changes in the telomeric DNA structure. Our results demonstrate a novel role of the S. pombe ATM homologues that is independent of chk1(+) and cds1(+).