Strain-specific differences in the effect of influenza A virus neuraminidase on vector-expressed hemagglutinin

作     者：Kaverin, NV Klenk, HD 

作者机构：Univ Marburg Inst Virol Marburg Germany 

出 版 物：《ARCHIVES OF VIROLOGY》 (病毒学文献)

年 卷 期：1999年第144卷第4期

页      面：781-786页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

主　　题：细胞系 长尾猴 黑 鸭 遗传载体 HN蛋白质/生物合成 HN蛋白质/遗传学 流感病毒A型/酶学 流感病毒A型/遗传学 流感病毒A型/生理学 神经氨酸酶/代谢 猴病毒40 种特异性 病毒复制 动物 

摘      要：In order to evaluate the efficiency of the removal of sialic acid residues from the influenza virus hemagglutinin by the viral neuraminidase in the course of the virus replication cycle, CV-1 cells expressing the hemagglutinin of H7 subtype from an SV40-based vector were superinfected with influenza virus strain A/Duck/Ukraine/63 (H3N8) or A/USSR/90/77 (H1N1). Vector-expressed hemagglutinin was immunoprecipitated from cell lysates and analyzed by polyacrylamide gel electrophoresis. The data indicate that the removal of sialic acid residues from the vector-expressed H7 hemagglutinin by N1 neuraminidase of A/USSR/90/77 virus in the course of the virus replication cycle is incomplete. The results are discussed in connection with previously published data showing that the low activity of NA in wild-type influenza virus results in incomplete removal of sialic acid residues from virion components.