A model for studying megakaryocyte development and biology

作     者：Murphy, GJ Leavitt, AD 

作者机构：Univ Calif San Francisco Dept Lab Med San Francisco CA 94143 USA Univ Calif San Francisco Dept Internal Med San Francisco CA 94143 USA 

出 版 物：《PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA》 (美国国家科学院汇刊)

年 卷 期：1999年第96卷第6期

页      面：3065-3070页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：NHLBI NIH HHS [P50 HL54476  P50 HL054476] Funding Source: Medline 

主　　题：细胞分化/遗传学 细胞系 细胞谱系/遗传学 鸡 巨核细胞/细胞学 小鼠 转基因 血小板糖蛋白GPⅡb-Ⅲa复合物 血小板糖蛋白GPⅠb-Ⅸ复合物/遗传学 动物 小鼠 

摘      要：The limited current understanding of megakaryocyte-lineage development and megakaryocyte biology is in large part because of a paucity of useful systems in which to conduct experiments. To overcome this problem, we have developed a transgenic mouse that uses the GP-Ib alpha: regulatory sequences to achieve megakaryocyte-lineage restricted expression of an avian retroviral receptor. Through the transgenic avian receptor, avian retroviruses can efficiently and selectively infect megakaryocyte-lineage cells in. vitro and in vivo. Serial infections can be performed to introduce and express multiple genes in the same cell. We have used this system to generate and characterize a pure population of primary CD41-positive megakaryocyte progenitors.