Isolation and characterisation of a ropy <i>Lactobacillus</i> strain producing the exopolysaccharide kefiran

作     者：Micheli, L Uccelletti, D Palleschi, C Crescenzi, V 

作者机构：Univ Roma La Sapienza Dept Chem I-00185 Rome Italy Univ Roma La Sapienza Dept Dev & Cell Biol Rome Italy 

出 版 物：《APPLIED MICROBIOLOGY AND BIOTECHNOLOGY》 (应用微生物学与生物技术)

年 卷 期：1999年第53卷第1期

页      面：69-74页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 0836[工学-生物工程] 071005[理学-微生物学] 10[医学] 

基　　金：Italian Ministry for Universities and Research MURST 

主　　题：乳杆菌属/分离和提纯 乳杆菌属/代谢 多糖类 细菌/生物合成 多糖类 细菌/化学 

摘      要：A capsular-polysaccharide-producing strain, LM-17, was isolated from kefir grains and was identified as a slime-forming, rod-shaped Lactobacillus. According to H-1- and C-13-NMR spectral data, the exopolysaccharide produced by the isolated bacterial strain is identical to the glucogalactan extracted from kefir grains and therefore known as kefiran. The kefiran produced was characterised by means of viscosity, optical rotatory power, circular dichroism and IR spectral measurements. A batch procedure was set up for the culture and extraction of the exopolysaccharide in laboratory conditions, resulting in a yield of 2 g/l purified kefiran from the culture supernatant of the LM-17 strain.