Patterned glass surfaces direct cell adhesion and process outgrowth of primary neurons of the central nervous system

作     者：Saneinejad, S Shoichet, MS 

作者机构：Univ Toronto Dept Chem Engn & Appl Chem Toronto ON M5S 3E5 Canada Univ Toronto Dept Chem Toronto ON M5S 3E5 Canada 

出 版 物：《JOURNAL OF BIOMEDICAL MATERIALS RESEARCH》 (生物医学材料研究杂志，B部分：应用生物材料)

年 卷 期：1998年第42卷第1期

页      面：13-19页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0836[工学-生物工程] 

主　　题：cell-substrate interaction cell adhesion cell repulsion guidance poly(ethylene glycol) hippocampal neurons patterning peptides 

摘      要：Glass surfaces were patterned with cell-adhesive regions of laminin adhesive peptides YIGSR, RGD, and IKVAV, and cell-repulsive regions of poly(ethylene glycol) (PEG). The patterns were created by sputter-coating titanium and then gold onto glass coverslips through electron microscope grids. Gold surfaces were modified with cysteine-terminated peptides to have approximately 450 fmol/cm(2) of peptide incorporated on the glass coverslips as determined with radiolabeled CGYIGSR. Amine-functionalized glass coverslips were prepared using an amine-functionalized silane and then further modified with PEG-aldehyde by a Schiff base reduction. All surfaces were characterized by X-ray photoelectron spectroscopy and water contact angles. Hippocampal neurons, plated from a serum-free medium, adhered preferentially to peptide-functionalized surfaces over PEG-modified surfaces. Cell adhesion and neurite outgrowth were limited to the peptide region, demonstrating that neurite outgrowth could be directed by a combination of cell-adhesive and cell-repulsive cues. (C) 1998 John Wiley & Sons, Inc.