Sphingosine-1-phosphate and lysophosphatidic acid trigger invasion of primitive hematopoietic cells into stromal cell layers

作     者：Yanai, N Matsui, N Furusawa, T Okubo, T Obinata, M 

作者机构：Tohoku Univ Inst Dev Aging & Canc Dept Cell Biol Aoba Ku Sendai Miyagi 9808575 Japan 

出 版 物：《BLOOD》 (血液)

年 卷 期：2000年第96卷第1期

页      面：139-144页

核心收录：

学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

主　　题：骨髓细胞/细胞学 细胞系 细胞 培养的 共同培养技术 培养基 造血干细胞/细胞学 造血干细胞/药物作用 造血干细胞/生理学 脂类/血液 溶血磷脂素类/药理学 小鼠 近交C57BL 信号传导/药物作用 信号传导/生理学 鞘氨醇/类似物和衍生物 鞘氨醇/药理学 间质细胞/细胞学 间质细胞/生理学 动物 牛 小鼠 

摘      要：A new primitive hematopoietic cell line (THS119), exhibiting Lin(-)/Sca-1(+)/c-Kit(+) a surface phenotype, grew and survived underneath stromal cells (TBR59). The ability of the THS119 cells to invade these stromal cell layers was dependent on the inclusion of serum in the culture medium, This was apparently due to a requirement for lipids contained in serum. Their invasion of the stromal cell layers in serum-free cultures could be triggered by addition of sphingosine-1-phosphate (S1P) or lysophosphatidic acid (LPA) and was dependent on both Rho- and Ras-signaling pathways. Between the 2 possible receptors of S1P and LPA, edg-1 and edg-2, expression of edg-2 only was found to be correlated with immaturity and/or invasive activity of the primitive hematopoietic cells. These results suggest the importance of specific lipids and their specific receptors on the invasive activity of primitive hematopoietic cells in the hematopoietic microenvironment. (Blood. 2000;96:139-144) (C) 2000 by The American Society of Hematology.