Cloning and expression of the pediocin operon in <i>Streptococcus thermophilus</i> and other lactic fermentation bacteria

作     者：Coderre, PE Somkuti, GA 

作者机构：USDA ARS Eastern Reg Res Ctr Wyndmoor PA 19038 USA 

出 版 物：《CURRENT MICROBIOLOGY》 (当今微生物学)

年 卷 期：1999年第39卷第5期

页      面：295-301页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

主　　题：细菌素类/生物合成 细菌素类/遗传学 细菌素类/药理学 克隆 分子 肠球菌 粪/遗传学 肠球菌 粪/代谢 大肠杆菌/遗传学 大肠杆菌/代谢 乳杆菌属/药物作用 乳球菌 乳/遗传学 乳球菌 乳/代谢 微生物敏感性试验 操纵子 片球菌属/遗传学 片球菌属/代谢 质粒/遗传学 链球菌属/药物作用 链球菌属/遗传学 链球菌属/代谢 

摘      要：Production of pediocin in Pediococcus acidilactici is associated with pMBR 1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors that contain all four structural genes, the chromosomal promoter STP2201 from Streptococcus thermophilus, and repA from the 2-kbp S. thermophilus plasmid pER8. The recombinant plasmid, pPC318, expressed and secreted active pediocin in Escherichia coli. Streptococcus thermophilus, Lactococcus lactis subsp. lactis, and Enterococcus faecalis::were electrotransformed with pPC418, a modified vector fitted with an erythromycin resistance tracking gene. Pediocin was produced and secreted in each of the lactic acid bacteria, and production was Stable for up to ten passages. The expression of pediocin in dairy fermentation microbes has important implications for bacteriocins as food preservatives in dairy products.