Mist1 expression is a common link among serous exocrine cells exhibiting regulated exocytosis

作     者：Pin, CL Bonvissuto, AC Konieczny, SF 

作者机构：Purdue Univ Dept Biol Sci W Lafayette IN 47907 USA 

出 版 物：《ANATOMICAL RECORD》 (解剖学记录)

年 卷 期：2000年第259卷第2期

页      面：157-167页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 071002[理学-动物学] 

基　　金：NIAMS NIH HHS [AR41115] Funding Source: Medline NIDDK NIH HHS [R01 DK055489, DK55489] Funding Source: Medline 

主　　题：basic helix-loop-helix E-box transcription acinar cells secretion 

摘      要：Mist1 is a basic helix-loop-helix transcription factor that represses E-box-mediated transcription. Previous studies have suggested that the Mist1 gene is expressed in a wide range of tissues, although a complete characterization of Mist1 protein accumulation in the adult organism has not been described. In an effort to identify specific cell types that contain the Mist1 protein, antibodies specific for Mist1 were generated and used in Western blot and immunohistochemical assays. Our studies show that the Mist1 protein is present in many different tissues but that it is restricted to cell types that are exclusively secretory in nature. Pancreatic acinar cells, serous or seromucous cells of the salivary glands, chief cells of the stomach, and secretory cells of the prostate and seminal vesicle show high levels of Mist1 protein, whereas nonserous exocrine cells, including the mucus-producing cells of the salivary glands, remain Mist1 negative. These results identify Mist1 as the first transcription factor that exhibits this unique serous-specific expression pattern and suggest that Mist1 may have a key role in establishing and maintaining a pathway responsible for the exocytosis of serous secretions. Anat Rec 259:157-167, 2000. (C) 2000 Wiley-Liss, Inc.