Characterisation of cellulose-binding proteins that are involved in the adhesion mechanism of <i>Fibrobacter intestinalis</i> DR7

作     者：Miron, J Forsberg, CW 

作者机构：Agr Res Org Volcani Ctr Inst Anim Sci Metab Unit IL-50250 Bet Dagan Israel Univ Guelph Dept Microbiol Guelph ON N1G 2W1 Canada 

出 版 物：《APPLIED MICROBIOLOGY AND BIOTECHNOLOGY》 (应用微生物学与生物技术)

年 卷 期：1999年第51卷第4期

页      面：491-497页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 0836[工学-生物工程] 071005[理学-微生物学] 10[医学] 

基　　金：Natural Sciences and Engineering Research Council of Canada, NSERC Ontario Ministry of Food and Agriculture, OMAF 

主　　题：细菌黏附/生理学 细菌蛋白质类/化学 细菌蛋白质类/分离和提纯 细菌蛋白质类/代谢 印迹法 蛋白质 纤维素/代谢 色谱法 高压液相 电泳 聚丙烯酰氨凝胶 糖基化 革兰氏阴性厌氧菌/代谢 革兰氏阴性厌氧菌/生理学 单糖类/分析 动物 兔 

摘      要：Cellulose-binding proteins (CBP) isolated from cell envelopes of the cellulolytic bacterium Fibrobacter intestinalis strain DR7 were studied in order to investigate the adhesion mechanism. The proteins were examined for their reaction with antibodies that specifically block bacterial adhesion, response to glycosylation staining and monosaccharide composition. To this end, the effect of some monosaccharides (CBP components) on blocking of DR7 adhesion to cellulose was determined. Previous study had shown the occurrence of 16 CBP in the outer membrane and periplasm of DR7, of which 6 had endoglucanase activity (Miron and Forsberg 1998). Data from the present study show that most of the 16 CBP of DR7, except for the 38-, 90- and 180-kDa proteins, are glycosylated. Rabbit antibodies that specifically block DR7 adhesion were prepared by affinity preabsorption of antiserum against wild-type DR7 with bacterial cells of its adherence-defective mutant (DR7-M). The preabsorbed antibodies reacted positively in Western blotting with glycosylated CBP of 225, 200, 150, 70, 45 and 38 kDa from the DR7 outer membrane, and reacted weakly with CBP of DR7-M. Modification of glycosidic residues attached to the CBP of DR7 by periodate oxidation prevented any reaction with the preabsorbed antibodies. Monosaccharide analysis by HPLC of isolated CBP from the outer membrane and periplasm of DR7 cells, showed that galactosamine, glucosamine, galacturonic acid, and glucuronic acid were the predominant monosaccharide components of CBP that can block the adhesion of DR7 cells to cellulose. It is suggested that some glycosylated residues of CBP may have a predominant role in the adhesion of DR7 to cellulose.