Biosynthesis and immunolocalization of Lewis a-containing <i>N</i>-glycans in the plant cell

作     者：Fitchette, AC Cabanes-Macheteau, M Marvin, L Martin, B Satiat-Jeunemaitre, B Gomord, V Crooks, K Lerouge, P Faye, L Hawes, C 

作者机构：Univ Rouen Fac Sci Transports Intracellulaires Lab CNRSESA 6037European Inst Peptide ResIFRMP 23 F-76821 Mt St Aignan France Univ Rouen Fac Sci CNRSESA 6014 IFRMP 23 F-76821 Mt St Aignan France Oxford Brookes Univ Res Sch Biol & Mol Sci Oxford OX3 0BP England CNRS UPR 40 Inst Sci Vegetales F-91198 Gif Sur Yvette France 

出 版 物：《PLANT PHYSIOLOGY》 (植物生理学)

年 卷 期：1999年第121卷第2期

页      面：333-343页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

主　　题：拟南芥属/超微结构 碳水化合物序列 表位/化学 真菌/细胞学 真菌/超微结构 糖蛋白类/生物合成 糖蛋白类/化学 高尔基体/超微结构 Lewis血型系统/化学 哺乳动物 分子序列数据 植物/细胞学 植物/超微结构 多糖类/分析 多糖类/生物合成 多糖类/化学 种特异性 动物 

摘      要：We recently demonstrated the presence of a new asparagine-linked complex glycan on plant glycoproteins that harbors the Lewis a (Le(a)), or Gal beta(1-3)[Fuc alpha(1-4)]GlcNAc, epitope, which in mammalian cells plays an important role in cell-to-cell recognition. Here we show that the monoclonal antibody JIM 84, which is widely used as a Golgi marker in light and electron microscopy of plant cells, is specific for the Le(a) antigen. This antigen is present on glycoproteins of a number of flowering and non-flowering plants, but is less apparent in the Cruciferae, the family that includes Arabidopsis. Le(a)-containing oligosaccharides are found in the Golgi apparatus, and our immunocytochemical experiments suggest that it is synthesized in the trans-most part of the Golgi apparatus. Le(a) epitopes are abundantly present on extracellular glycoproteins, either soluble or membrane bound, but are never observed on vacuolar glycoproteins. Double-labeling experiments suggest that vacuolar glycoproteins do not bypass the late Golgi compartments where Le(a) is built, and that the absence of the Lea epitope from vacuolar glycoproteins is probably the result of its degradation by glycosidases en route to or after arrival in the vacuole.