Identification of <i>Lactobacillus</i> isolates from the gastrointestinal tract, silage, and yoghurt by 16S-23S rRNA gene intergenic spacer region sequence comparisons

作     者：Tannock, GW Tilsala-Timisjarvi, A Rodtong, S Ng, J Munro, K Alatossava, T 

作者机构：Univ Otago Dept Microbiol Dunedin New Zealand Univ Oulu Dept Biol Oulu Finland Univ Oulu REDEC Kajaani Biotechnol Lab Sotkamo Finland Suranaree Univ Technol Sch Microbiol Nakhon Ratchasima Thailand 

出 版 物：《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 (应用与环境微生物学)

年 卷 期：1999年第65卷第9期

页      面：4264-4267页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 0836[工学-生物工程] 071005[理学-微生物学] 10[医学] 

主　　题：细菌分型技术 DNA 细菌/遗传学 DNA 核糖体/遗传学 消化系统/微生物学 基因 rRNA 乳杆菌属/分类 乳杆菌属/遗传学 乳杆菌属/分离和提纯 聚合酶链反应/方法 RNA 核糖体 16S/遗传学 RNA 核糖体 23S/遗传学 序列分析 DNA 青贮饲料/微生物学 酸乳/微生物学 人类 

摘      要：Lactobacillus isolates were identified by PCR amplification and sequencing of the region between the 16S and 23S rRNA genes (spacer region). The sequences obtained from the isolates were compared to those of reference strains held in GenBank A similarity of 97.5% or greater was considered to provide identification. To check the reliability of the method, the V2-V3 region of the 16S rRNA gene was amplified and sequenced in the case of isolates whose spacer region sequences were less than 99% similar to that of a reference strain. Confirmation of identity was obtained in all instances. Spacer region sequencing provided rapid and accurate identification of Lactobacillus isolates obtained from gastrointestinal, yoghurt, and silage samples. It had, an advantage over 16S V2-V3 sequence comparisons because it distinguished between isolates of Lactobacillus casei and Lactobacillus rhamnosus.