Effects of <i>in vivo</i> administration of adamantylamide dipeptide on bone marrow granulocyte-macrophage hemopoietic progenitor cells (GM-CFC) and on ability of serum of the treated mice to stimulate GM-CFC colony formation <i>in vitro</i>:: Comparison with muramyl dipeptide and glucan

作     者：Vacek, A Hofer, M Masek, K 

作者机构：Acad Sci Czech Republic Inst Biophys CS-61265 Brno Czech Republic Acad Sci Czech Republic Inst Pharmacol Prague 14220 4 Czech Republic 

出 版 物：《IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY》 (Immunopharmacol. Immunotoxicol.)

年 卷 期：1999年第21卷第1期

页      面：1-14页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

主　　题：乙酰胞壁酰-丙氨酰-异谷酰胺/免疫学 乙酰胞壁酰-丙氨酰-异谷酰胺/药理学 佐剂 免疫/药理学 金刚烷胺/类似物和衍生物 金刚烷胺/免疫学 金刚烷胺/药理学 骨髓细胞/药物作用 骨髓细胞/免疫学 细胞 培养的 交叉 遗传 二肽类/免疫学 二肽类/药理学 葡聚糖类/免疫学 葡聚糖类/药理学 粒细胞/药物作用 粒细胞/免疫学 造血干细胞/药物作用 造血干细胞/免疫学 免疫血清/免疫学 巨噬细胞/药物作用 巨噬细胞/免疫学 小鼠 近交C57BL 小鼠 近交CBA 动物 小鼠 

摘      要：Adamantylamide dipeptide (AdDP), muramyl dipeptide (MDP), and glucan were shown to increase significantly the numbers of granulocyte-macrophage hemopoietic progenitor cells (GM-CFC) in the bone marrow of mice. However, whereas the sera of mice given MDP or glucan were found to stimulate the growth of colonies from GM-CFC in vitro, i.e. to produce a colony-stimulating activity (CSA), administration of AdDP did not lead to this effect. Nevertheless, when serum of mice given AdDP was added to the cultures concomitantly with a suboptimal concentration of mouse interleukin-3 (mIL-3), a broad spectrum hemopoietic stimulator, counts of colonies from GM-CFC were significantly increased, and accelerated growth of the colonies was found as well. This property of AdDP, i.e. its ability to exhibit co-stimulating activity (CoSA) without being able to exhibit CSA, suggests that AdDP acts in hemopoietic tissues differently as compared with the two other immunomodulators studied. It can be hypothesized that the action of AdDP is more specific when compared with its natural related compound, MDP, as well as with glucan. Our findings prove the possibility td stimulate by AdDP the granulopoietic compartment of hemopoiesis and are in agreement with previous observations concerning the absence of systemic side effects of AdDP. Both these qualities of AdDP may be advantageous when pondering over contingent clinical utilization of AdDP as hemopoietic stimulator.