Human transcription factor hTAF_II150 (CIF150) is involved in transcriptional regulation of cell cycle progression

作     者：Martin, J Halenbeck, R Kaufmann, J 

作者机构：Chiron Corp Chiron Technol Emeryville CA 94608 USA 

出 版 物：《MOLECULAR AND CELLULAR BIOLOGY》 (分子生物学与细胞生物学)

年 卷 期：1999年第19卷第8期

页      面：5548-5556页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

主　　题：细胞周期/遗传学 细胞周期/生理学 细胞系 色谱法 凝胶 细胞周期蛋白A/生物合成 细胞周期蛋白A/遗传学 细胞周期蛋白B/生物合成 细胞周期蛋白B/遗传学 细胞周期蛋白B1 DNA结合蛋白质类/生理学 果蝇蛋白质类 G2期 基因表达调控 基因表达调控 肿瘤 基因打靶 基因 报告 HeLa细胞 大分子物质 中期 肿瘤蛋白质类/生物合成 肿瘤蛋白质类/遗传学 RNA聚合酶Ⅱ/代谢 逆转录聚合酶链反应 底物特异性 TATA结合蛋白质相关因子类 转录因子TFIID 转录因子/生理学 转录因子 TFⅡ类/代谢 转录 遗传/生理学 转染 人类 

摘      要：Here we present evidence that CIF150 (hTAF(II)150), the human homolog: of Drosophila TAF(II)150, plays an important and selective role in establishing gene expression patterns necessary for progression through the cell cycle. Gel filtration experiments demonstrate that CIF150 (hTAF(II)150) seems to be less tightly associated with human transcription factor IID than hTAF(II)130 is associated with hTAF(II)250. The transient functional knockout of CIF150 (hTAF(II)150) protein led to cell cycle arrest at the G(2)/M transition in mammalian cell lines. PCR display analysis with the RNA derived from CIF150-depleted cells indicated that CIF150 (hTAF(II)150) is required for the transcription of only a subset of RNA polymerase II genes. CIF150 (hTAF(II)150) directly stimulated cyclin B1 and cyclin ii transcription in cotransfection assays and in vitro assays, suggesting that the expression of these genes is dependent on CIF150 (hTAF(II)150) function. We defined a CIF150 (hTAF(II)150) consensus binding site and demonstrated that a CIF150-responsive cis element is present in the cyclin B1 core promoter. These results suggest that one function of CIF150 (hTAF(II)150) is to select specific RNA polymerase II core promoter elements involved in cell cycle progression.