The quiescent-cell expression system for protein synthesis in <i>Escherichia coli</i>

作     者：Rowe, DCD Summers, DK 

作者机构：Univ Cambridge Dept Genet Cambridge CB2 3EH England 

出 版 物：《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 (应用与环境微生物学)

年 卷 期：1999年第65卷第6期

页      面：2710-2715页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 0836[工学-生物工程] 071005[理学-微生物学] 10[医学] 

主　　题：细菌蛋白质类/生物合成 氯霉素O-乙酰转移酶/生物合成 DNA结合蛋白质类/遗传学 DNA结合蛋白质类/代谢 大肠杆菌/遗传学 大肠杆菌/生长和发育 大肠杆菌/代谢 基因表达调控 细菌 乳糖操纵子 甲硫氨酸/代谢 质粒 RNA 细菌/遗传学 RNA 细菌/代谢 硫放射性同位素/代谢 β半乳糖苷酶类/生物合成 

摘      要：The quiescent-cell expression system is a radical alternative to conventional fermentation for protein overproduction in Escherichia coli. It is dependent on the controlled overexpression of a small RNA called Red in hns mutant, strains to generate nongrowing, quiescent cells which are not nutrient limited. Quiescent cells no longer produce biomass and have their metabolic resources channelled toward the expression of plasmid-based genes. The. biosynthetic capacity of the system is demonstrated by its ability to express chloramphenicol acetyltransferase to more than. 40% of total cell protein. Quiescent cells may provide an ideal environment for the expression of toxic as well as benign proteins.