Cytomegalovirus-encoded β chemokine promotes monocyte-associated viremia in the host

作     者：Saederup, N Lin, YC Dairaghi, DJ Schall, TJ Mocarski, ES 

作者机构：Stanford Univ Sch Med Dept Microbiol & Immunol Stanford CA 94305 USA ChemoCentryx San Carlos CA 94070 USA 

出 版 物：《PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA》 (美国国家科学院汇刊)

年 卷 期：1999年第96卷第19期

页      面：10881-10886页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：NIAID NIH HHS [R01 AI030363, R01 AI30363, R01 AI28341] Funding Source: Medline NIGMS NIH HHS [T32 GM07328] Funding Source: Medline 

主　　题：氨基酸序列 钙/代谢 炎症趋化因子类/遗传学 炎症趋化因子类/代谢 趋化因子 CC/遗传学 趋化因子 CC/生理学 巨细胞病毒/遗传学 巨细胞病毒/代谢 小鼠 近交BALBC 模型 生物学 模型 遗传学 分子序列数据 单核细胞/代谢 单核细胞/病毒学 腹膜/代谢 腹膜/病毒学 重组 遗传 序列同源性 氨基酸 时间因素 病毒蛋白质类 病毒血症/遗传学 病毒血症/代谢 动物 人类 小鼠 

摘      要：Chemokine homologs are encoded by many large DNA viruses, suggesting that they contribute to control of host leukocyte transmigration and trafficking during viral infection. Murine cytomegalovirus carries a CC (beta) chemokine homolog gene giving rise to two related proteins, murine cytomegalovirus chemokine 1 and 2 (MCK-1 and MCK-2). MCK-1 peptide was found to induce calcium signaling and adherence in murine peritoneal macrophages. Cells bearing human chemokine receptor CCR3 and the human macrophage THP1 cell line were responsive to MCK-1. This pattern suggested that MCK-1 might act as an agonist, promoting leukocyte trafficking during viral infection. Consistent with this prediction, MCK-1/MCK-2 mutant viruses exhibit dramatically reduced peak levels of monocyte-associated viremia in experimentally infected mice. Thus, MCK-1/MCK-2 appears to promote host leukocyte migration to initial sites of infection and may be responsible for attracting monocytes or macrophages that efficiently disseminate virus in the host.