液-质联用法测定小鼠体内二苯乙烯苷代谢物

作     者：孙江浩 张兰桐 王春英 袁志芳 卢兴红 

作者机构：河北医科大学药学院药物分析教研室河北石家庄050017 

出 版 物：《药学学报》 (Acta Pharmaceutica Sinica)

年 卷 期：2003年第38卷第12期

页      面：968-970页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：二苯乙烯苷 液-质联用 药物代谢 葡糖醛酸 

摘      要：Aim To study the metabolite of stilbene glucoside in the Chinese traditional medicine Polygonum multiflornm in mice and elucidate its chemical structure by liquid chromatography tandem-mass spectrometry. Methods The stilbene glucoside was injected into the tail vein of mice. Blood samples were collected from artery in the eyepit. The methanol-protein-precipitated plasma sample was introduced into the liquid chromatography-tandem mass spectrometer directly. The analytical column was C_ 18 column (250 mm×4.6 mm ID, 5 μm). The mobile phase consisted of acetonitrile-methanol-water-formic acid (15∶18∶66∶1) for ES +, acetonitrile-methanol-water (15∶18∶67) for ES -. The UV detection wavelength was set at 320 nm. The mass ion source type was ESI. HV capillary was 3 kV. The dry gas was nitrogen gas and the flow rate was set at 318 L·h -1 . The ion source temperature was 150 ℃. Results The stilbene glucoside and its metabolite were separated completely under the chromatography condition. The ions at m/z 600 and m/z 605 were detected under positive ion polarity while the ions at m/z 581 and m/z 402 were detected under negative ion polarity. Conclusion It was proposed that the metabolite of stilbene glucoside injected in vein was its glucuronide conjugate.