Immunohistochemical localization of thrombomodulin in the stratified epithelium of the rat is restricted to the keratinizing epidermis

作     者：Daimon, T Nakano, M 

作者机构：Teikyo Univ Sch Med Dept Anat Itabashi Ku Tokyo 1738605 Japan Mitsubishi Gas Chem Co Niigata Res Lab Niigata 95031 Japan 

出 版 物：《HISTOCHEMISTRY AND CELL BIOLOGY》 (组织化学和细胞生物学)

年 卷 期：1999年第112卷第6期

页      面：437-442页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0804[工学-仪器科学与技术] 0901[农学-作物学] 090102[农学-作物遗传育种] 

主　　题：细胞膜/代谢 表皮/代谢 表皮/超微结构 上皮/代谢 上皮/超微结构 免疫组织化学 角蛋白细胞/代谢 角蛋白细胞/超微结构 显微镜检查 免疫电子 大鼠 Wistar 血栓调节蛋白/代谢 动物 女(雌)性 男(雄)性 大鼠 

摘      要：The expression and function of thrombomodulin (TM), an endothelial cofactor protein for thrombin-mediated protein C activation, in the epithelium are not fully characterized. This report describes the distribution and localization of TM in the various types of epithelia in the rat by light and electron microscopic immunocytochemistry. TM showed a limited distribution and was expressed by the keratinizing stratified epithelia of the skin, tongue, and esophagus, but was not present on the non-keratinizing epithelia of the vagina, ureter, trachea, stomach, or gut. An identical pattern of TM expression was seen in mucocutaneous junctions, transitional zones from a non-keratinizing stratified epithelium to a keratinizing epithelium at the edge of the eyelid and in the anal canal. As the keratinization of the stratified epithelia proceeded, the staining intensity increased in the transitional zones. Within the keratinizing stratified epithelia, TM staining was limited to the keratinocytes of the spinous layer, the spinous cells. The subcellular localization of TM on the spinous cells was restricted to the plasma membrane facing the intercellular spaces. TM was not detectable on the desmosomes or the two membranes making up the junction, presumably the nexus. The functional significance of TM in keratinizing epithelia is discussed.