The morphology of <i>Ichthyophonus</i> sp in their mugilid hosts (Pisces: Teleostei) and following cultivation in vitro.: A light and electron microscopy study

作     者：Franco-Sierra, A Alvarez-Pellitero, P 

作者机构：CSIC Inst Acuicultura Torre Sal E-12595 Ribera De Cabanes Castellon Spain 

出 版 物：《PARASITOLOGY RESEARCH》 (寄生虫学研究)

年 卷 期：1999年第85卷第7期

页      面：562-575页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 09[农学] 

基　　金：Comisión Interministerial de Ciencia y Tecnología  CICYT 

主　　题：培养基 鱼疾病/微生物学 氢离子浓度 显微镜检查 电子 鲈形目/寄生虫学 孢子 真菌/超微结构 接合菌亚纲/生长和发育 接合菌亚纲/分离和提纯 接合菌亚纲/超微结构 动物 牛 

摘      要：The morphology of Ichthyophonus sp., a parasite of Mugil capito and Liza saliens, was investigated by light and transmission electron microscopy. The most frequent stage found in the fish hosts was the multinucleate spore, though germinating stages, hyphae, and endospores were also found. Different development patterns were observed in the media assayed for in vitro culture. Optimal growth and development were obtained in Eagle s minimum essential medium (MEM) supplemented with 10% fetal bovine serum at pH 7. Ultrastructural features of multinucleate spores, both in the fish host and in culture, were a fibrillar thick wall and an electron-lucent matrix, with large glycogen granules, some electron-dense bodies, large vacuoles, lipid inclusions, and endoplasmic reticulum mainly appearing among the nuclei. Mitochondria with scarce tubulovesicular cristae were observed in the different stages, mainly near the wall and the germinating sites. Condensed heterochromatin was rarely seen. A nucleus-associated organelle (NAO) was frequently observed, and dictyosome cisternae and vesicles appeared in its vicinity.