Hydrogen peroxide from the oxidative burst is neither necessary nor sufficient for hypersensitive cell death induction, phenylalanine ammonia lyase stimulation, salicylic acid accumulation, or scopoletin consumption in cultured tobacco cells treated with elicitin

作     者：Dorey, S Kopp, M Geoffroy, P Fritig, B Kauffmann, S 

作者机构：Univ Strasbourg 1 CNRS Inst Biol Mol Plantes F-67084 Strasbourg France 

出 版 物：《PLANT PHYSIOLOGY》 (植物生理学)

年 卷 期：1999年第121卷第1期

页      面：163-171页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

主　　题：藻蛋白质类/药理学 细胞死亡/药物作用 细胞 培养的 酶激活/药物作用 糖蛋白类/药理学 过氧化氢/代谢 苯丙氨酸氨裂合酶/代谢 疫霉属 植物疾病/化学诱导 植物生长调节物/药理学 植物叶/细胞学 植物叶/药物作用 植物叶/酶学 植物叶/代谢 植物 有毒 呼吸爆发/药物作用 水杨酸/代谢 东莨菪亭/代谢 烟草/细胞学 烟草/药物作用 烟草/酶学 烟草/代谢 

摘      要：H2O2 from the oxidative burst, cell death, and defense responses such as the production of phenylalanine ammonia lyase (PAL), salicylic acid (SA), and scopoletin were analyzed in cultured tobacco (Nicotiana tabacum) cells treated with three proteinaceous elicitors: two elicitins (alpha-megaspermin and beta-megaspermin) and one glycoprotein. These three proteins have been isolated from Phytophthora megasperma H20 and have been previously shown to be equally efficient in inducing a hypersensitive response (HR) upon infiltration into tobacco leaves. However, in cultured tobacco cells these elicitors exhibited strikingly different biological activities. beta-Megaspermin was the only elicitor that caused cell death and induced a strong, biphasic H2O2 burst. Both elicitins stimulated PAL activity similarly and strongly, while the glycoprotein caused only a slight increase. Only elicitins induced SA accumulation and scopoletin consumption, and beta-megaspermin was more efficient. To assess the role of H2O2 in HR cell death and defense response expression in elicitin-treated cells, a gain and loss of function strategy was used. Our results indicated that H2O2 was neither necessary nor sufficient for HR cell death, PAL activation, or SA accumulation, and that extracellular H2O2 was not a direct cause of intracellular scopoletin consumption.