Interaction between astrocytes and adult subventricular zone precursors stimulates neurogenesis

作     者：Lim, DA Alvarez-Buylla, A 

作者机构：Rockefeller Univ New York NY 10021 USA 

出 版 物：《PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA》 (美国国家科学院汇刊)

年 卷 期：1999年第96卷第13期

页      面：7526-7531页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：National Institute of Neurological Disorders and Stroke [R01NS028478] Funding Source: NIH RePORTER NIGMS NIH HHS [T32 GM007739, GM07739] Funding Source: Medline NINDS NIH HHS [R37 NS028478, R01 NS028478, NS28478] Funding Source: Medline 

主　　题：星形细胞/细胞学 星形细胞/生理学 细胞间通讯/生理学 细胞分化/生理学 细胞运动/生理学 细胞 培养的 神经元/细胞学 神经元/生理学 前脑/细胞学 前脑/生理学 成年人 人类 

摘      要：Neurogenesis continues in the mammalian subventricular zone (SVZ) throughout life. However, the signaling and cell-cell interactions required for adult SVZ neurogenesis are not known. In vivo, migratory neuroblasts (type A cells) and putative precursors (type C cells) are in intimate contact with astrocytes (type B cells). Type B cells also contact each other. We reconstituted SVZ cell-cell interactions in a culture system free of serum or exogenous growth factors, Culturing dissociated postnatal or adult SVZ cells on astrocyte monolayers-but not other substrates-supported extensive neurogenesis similar to that observed in vivo. SVZ precursors proliferated rapidly on astrocytes to form colonies containing up to 100 type A neuroblasts. By fractionating the SVZ cell dissociates with differential adhesion to immobilized polylysine, we show that neuronal colony-forming precursors were concentrated in a fraction enriched for type B and C cells. Pure type A cells could migrate in chains but did not give rise to neuronal colonies. Because astrocyte-conditioned medium alone was not sufficient to support SVZ neurogenesis, direct cell-cell contact between astrocytes and SVZ neuronal precursors may be necessary for the production of type A cells.