Increased <i>c-fos</i> mRNA expression by human fibroblasts contracting stressed collagen matrices

作     者：Rosenfeldt, H Lee, DJ Grinnell, F 

作者机构：Univ Texas SW Med Sch Dept Cell Biol & Neurosci Dallas TX 75235 USA 

出 版 物：《MOLECULAR AND CELLULAR BIOLOGY》 (分子生物学与细胞生物学)

年 卷 期：1998年第18卷第5期

页      面：2659-2667页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：NIAMS NIH HHS [P30 AR041940, AR41940] Funding Source: Medline NIGMS NIH HHS [GM31321, R37 GM031321, R01 GM031321] Funding Source: Medline 

摘      要：We studied early changes in gene expression during fibroblast contraction of stressed collagen matrices. The level of c-fos mRNA increased dramatically and peaked 50 to 60 min after matrix contraction was initiated. This response did not require serum and could not be accounted for simply by disruption of the actin cytoskeleton. Increased c-fos mRNA levels required Ca2+ influx but not the cyclic AMP or extracellular signal-regulated kinase (ERK 1/2) signaling pathways, both of which are activated when fibroblasts contract stressed collagen matrices. The levels of two other immediate-early genes, fosb and c-jun, also increased transiently after fibroblast contraction, whereas the levels of fra-1, fra-2, c-myc, and the transcription factor NF-kappa B remained the same, indicating that fibroblast contraction caused changes in a selective group of genes. The increase in c-fos mRNA during contraction of stressed collagen matrices may reflect a unique role for c-fos in mechano-regulated events at the end of wound repair.