<i>sgk</i> is an aldosterone-induced kinase in the renal collecting duct -: Effects on epithelial Na<SUP>+</SUP> channels

作     者：Náray-Fejes-Tóth, A Canessa, C Cleaveland, ES Aldrich, G Fejes-Tóth, G 

作者机构：Dartmouth Med Sch Dept Physiol Lebanon NH 03756 USA Yale Univ Sch Med Dept Cellular & Mol Physiol New Haven CT 06520 USA 

出 版 物：《JOURNAL OF BIOLOGICAL CHEMISTRY》 (生物化学杂志)

年 卷 期：1999年第274卷第24期

页      面：16973-16978页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：NHLBI NIH HHS [HL56163] Funding Source: Medline NIDDK NIH HHS [DK41841, DK39523] Funding Source: Medline 

主　　题：醛固酮/生物合成 醛固酮/药理学 阿米洛利/药理学 氨基酸序列 雄甾烷醇类/药理学 导电性 酶诱导 即早蛋白质类 肾小管 集合/药物作用 肾小管 集合/酶学 分子序列数据 核蛋白质类 蛋白质丝氨酸苏氨酸激酶/生物合成 蛋白质丝氨酸苏氨酸激酶/遗传学 RNA 信使/生物合成 受体 盐皮质激素/生物合成 重组蛋白质类/代谢 序列同源性 氨基酸 动物 兔 

摘      要：The early phase of the stimulatory effect of aldosterone on sodium reabsorption in renal epithelia is thought to involve activation of apical sodium channels. However, the genes initiating this effect are unknown. We used a combination of polymerase chain reaction-based subtractive hybridization and differential display techniques to identify aldosterone-regulated immediate early genes in renal mineralocorticoid target cells. We report here that aldosterone rapidly increases mRNA levels of a putative Ser/Thr kinase, sgk (or serum- and glucocorticoid-regulated kinase), in its native target cells, i.e. in cortical collecting duct cells. The effect occurs within 30 min of the addition of aldosterone, is mediated through mineralocorticoid receptors, and does not require de novo protein synthesis. The full-length sequences of rabbit and mouse sgk cDNAs were determined. Both cDNAs show significant homology to rat and human sgk (88-94% at the nucleotide level, and 96-99% at the amino acid level). Coexpression of the mouse sgk in Xenopus oocytes with the three subunits of the epithelial Na+ channel results in a significantly enhanced Na+ current. These results suggest that sgk is an immediate early aldosterone-induced gene, and this protein kinase plays an important role in the early phase of aldosterone-stimulated Na+ transport.