Haemoglobin (<i>Erythrogen</i>™)-enhanced post-thaw growth of cryopreserved cells

作     者：Azhakanandam, K Anthony, P Lowe, KC Davey, MR Power, JB 

作者机构：Univ Nottingham Dept Life Sci Nottingham NG7 2RD England 

出 版 物：《ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY》 (Artif. Cells Blood Substitutes Immobilization Biotechnol.)

年 卷 期：1999年第27卷第2期

页      面：163-169页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1007[医学-药学(可授医学、理学学位)] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0836[工学-生物工程] 100403[医学-营养与食品卫生学] 10[医学] 

主　　题：细胞 培养的 低温保存/方法 血红蛋白类/药理学 水稻/细胞学 水稻/生长和发育 

摘      要：Supplementation of semi-solid R2 culture medium with a commercial bovine haemoglobin (Hb) solution (Erythrogen(TM)) at 1:50-1:500 (v:v), had beneficial effects on the growth, following cryopreservation, of cells of the Indica rice, Oryza sativa cv. Pusa Basmati 1. The mean absorbance, as assessed by triphenyl tetrazolium chloride reduction, of rice cells at 8 d post-thawing, was increased by up to 60% (P 0.05), compared to cells recovered in the absence of Hb. Erythrogen(TM) (1:50-1:500 v:v) promoted an increase in biomass, of up to 25% over control (P 0.05), at 24 d post-thawing. Cell suspensions, re-established by transfer to liquid medium of cells initially thawed and cultured with Erythrogen(TM) for 24 d, exhibited increased (up to 2-fold) growth rates over a subsequent 20-d period, compared to cells recovered without Hb.