Isolation of a proliferation inhibitor factor from uterine myomatosis fibroblasts

作     者：Sánchez-Sánchez, L Chávez, MA Manjarrez, A López, R Zenteno, E Weiss-Steider, B 

作者机构：Natl Autonomous Univ Mexico Fac Estudios Super Zaragoza Lab Biol Celular & Mol Canc Mexico City 04510 DF Mexico Natl Autonomous Univ Mexico Fac Med Dept Salud Publ Mexico City 04510 DF Mexico Natl Autonomous Univ Mexico Fac Med Lab Inmunol Dept Bioquim Mexico City 04510 DF Mexico 

出 版 物：《PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY》 (Prep. Biochem. Biotechnol.)

年 卷 期：1999年第29卷第1期

页      面：23-33页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 0836[工学-生物工程] 

主　　题：印迹法 蛋白质 细胞计数 色谱法 凝胶 剂量效应关系 药物 表皮生长因子/药理学 成纤维细胞/化学 龙胆紫/分析 生长抑制物/分离和提纯 干扰素Ⅱ型/药理学 白细胞介素1/药理学 平滑肌瘤/化学 肿瘤蛋白质类/分离和提纯 转化生长因子β/药理学 肿瘤细胞 培养的 肿瘤坏死因子α/药理学 子宫肿瘤/化学 女(雌)性 人类 

摘      要：In this work, we report the isolation of a factor from the culture supernatant of confluent fibroblasts from human cervix with the diagnosis of uterine myomatosis. This factor possesses the capacity to inhibit the proliferation of normal fibroblasts. The proliferation inhibitor factor (PIF) was purified from the culture supernatant by precipitation with 80% ammonium sulfate, and by molecular sieve chromatography. Our results indicate that PIF is a protein of 23 kDa, which is highly sensitive to trypsin treatment, and is thermolabile, since temperatures equal to, or above, 60 degrees C eliminate the protein activity in 15 to 20 min. Western blot analyses identified no cross reactions of the purified PIF with TGF-alpha, TNF alpha, IFN gamma, or IL-1 beta, suggesting that PIF is a new protein belonging to the group of factors secreted by fibroblasts able to inhibit cellular proliferation.